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[Establishment of a DNA bank of human origin].

[Establishment of a DNA bank of human origin].

Authors :
Rahuel C
Dandieu S
Chaussé AM
Mornet E
Rouger P
Cartron JP
Salmon C
Lucotte G
Source :
Revue francaise de transfusion et immuno-hematologie [Rev Fr Transfus Immunohematol] 1983 Nov; Vol. 26 (5), pp. 539-52.
Publication Year :
1983

Abstract

High molecular weight DNA extracted from 50 unrelated parisian individuals constitutes a "DNA-thèque" which can be used at the population level. This procedure allows preparation from a variety of tissues such as liver, placenta and leucocytes. The "quality" of the DNA obtained (about 50 Kb, RNA and protein free) and the efficiency of the method (in terms of the yield of DNA obtained) are considered. Blood is the easiest material to obtain and permits the obtaining of a reasonable amount of human and primate DNA, particularly when familial investigation is planned for polymorphism studies. One of the objects of this article is to encourage others to make use of our bank, for studies in population and evolutionary genetics.

Details

Language :
French
ISSN :
0338-4535
Volume :
26
Issue :
5
Database :
MEDLINE
Journal :
Revue francaise de transfusion et immuno-hematologie
Publication Type :
Academic Journal
Accession number :
6198708
Full Text :
https://doi.org/10.1016/s0338-4535(83)80123-8