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[Establishment of a DNA bank of human origin].
[Establishment of a DNA bank of human origin].
- Source :
-
Revue francaise de transfusion et immuno-hematologie [Rev Fr Transfus Immunohematol] 1983 Nov; Vol. 26 (5), pp. 539-52. - Publication Year :
- 1983
-
Abstract
- High molecular weight DNA extracted from 50 unrelated parisian individuals constitutes a "DNA-thèque" which can be used at the population level. This procedure allows preparation from a variety of tissues such as liver, placenta and leucocytes. The "quality" of the DNA obtained (about 50 Kb, RNA and protein free) and the efficiency of the method (in terms of the yield of DNA obtained) are considered. Blood is the easiest material to obtain and permits the obtaining of a reasonable amount of human and primate DNA, particularly when familial investigation is planned for polymorphism studies. One of the objects of this article is to encourage others to make use of our bank, for studies in population and evolutionary genetics.
- Subjects :
- Animals
Callithrix
DNA blood
DNA isolation & purification
Female
Genetics, Population
Humans
Lemuridae
Leukocytes analysis
Liver analysis
Male
Molecular Weight
Pan troglodytes
Papio
Pedigree
Placenta analysis
Proteins analysis
Proteins genetics
RNA analysis
RNA genetics
Tissue Preservation
DNA genetics
Tissue Banks
Subjects
Details
- Language :
- French
- ISSN :
- 0338-4535
- Volume :
- 26
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Revue francaise de transfusion et immuno-hematologie
- Publication Type :
- Academic Journal
- Accession number :
- 6198708
- Full Text :
- https://doi.org/10.1016/s0338-4535(83)80123-8