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Electrophoretic elution of nucleic acids from acrylamide and agarose gels.
- Source :
-
Journal of biochemical and biophysical methods [J Biochem Biophys Methods] 1983 Jul; Vol. 7 (4), pp. 277-84. - Publication Year :
- 1983
-
Abstract
- A simple method for electrophoretic elution of nucleic acids from gel slices is described. The procedure utilizes a standard tube gel system and can be completed in as little as one hour. Nucleic acids are recovered in a small volume with almost 100% efficiency. The procedure is applicable equally to acrylamide and agarose gels, and small as well as large RNA and DNA molecules. The eluted nucleic acids are essentially undegraded and are suitable for a variety of structural and biological analyses.
Details
- Language :
- English
- ISSN :
- 0165-022X
- Volume :
- 7
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of biochemical and biophysical methods
- Publication Type :
- Academic Journal
- Accession number :
- 6194199
- Full Text :
- https://doi.org/10.1016/0165-022x(83)90052-0