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Cysteamine and prostaglandin F2 beta stimulate rat gastric mucin release.
- Source :
-
Gastroenterology [Gastroenterology] 1983 Feb; Vol. 84 (2), pp. 306-13. - Publication Year :
- 1983
-
Abstract
- Gastric mucin glycoproteins form an adherent gel over the surface epithelium that is thought to protect the stomach against chemical and physical damage. The purpose of this study was to measure the release of mucin glycoproteins from rat stomach after treatment with cysteamine and prostaglandin F2 beta, two structurally unrelated drugs that have been shown to protect the stomach against the noxious effects of alcohol and other damaging agents. Gastric mucin was separated into soluble (washout) and insoluble (adherent) phases before colorimetric quantitation of total mucin, protein-bound hexose, and sialic acid. Cysteamine produced a dose-dependent increase in release of soluble and gel mucin. Prostaglandin F2 beta caused a dose-dependent release of hexose-containing mucin but had no effect on sialic acid-containing glycoproteins. Sepharose 4B chromatography of both the soluble and adherent mucus revealed that greater than 90% was a high molecular weight glycoprotein fraction. N-Ethylmaleimide, a known inhibitor of cytoprotection by cysteamine, had no effect on mucin secretion. Similarly, indomethacin inhibited mucin secretion by cysteamine but did not significantly influence cytoprotection. Thus the secretion of mucin by cytoprotective agents is unlikely by itself to explain the ability of the stomach to resist chemical or physical damage.
- Subjects :
- Animals
Dose-Response Relationship, Drug
Ethanol pharmacology
Ethylmaleimide pharmacology
Female
Gastric Mucosa metabolism
Glycoproteins metabolism
Indomethacin pharmacology
Mucus metabolism
Pepsin A metabolism
Rats
Rats, Inbred Strains
Stimulation, Chemical
Cysteamine pharmacology
Gastric Mucins metabolism
Gastric Mucosa drug effects
Prostaglandins F pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0016-5085
- Volume :
- 84
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 6184259