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Human T cell lines with antigen specificity and helper activity.
- Source :
-
European journal of immunology [Eur J Immunol] 1982 Jun; Vol. 12 (6), pp. 468-74. - Publication Year :
- 1982
-
Abstract
- Human T blasts, obtained by stimulation of peripheral blood mononuclear cells (PBM) with tetanus toxoid, diphtheria toxoid or Candida albicans, were expanded in long-term culture using alternate periods of antigen restimulation and growth in media containing interleukin 2. The cells gave a proliferative response only to the antigen originally used for stimulation. Such as response was strictly dependent upon the presence of autologous but not of allogeneic mitomycin C-treated mononuclear cells. When added to autologous PBM depleted of E-rosetting cells together with the specific antigen, the T blasts induced a polyclonal proliferation and differentiation of B cells. Allogeneic B cells were activated by antigen-stimulated T blasts only in the presence of irradiated mononuclear cells autologous to the responding T blasts. The above responses seemed not to be regulated solely by the release of soluble factors; apparently cell to cell interactions had to take place to obtain an efficient B cell activation.
- Subjects :
- Antibody-Producing Cells immunology
Antigens, Surface immunology
B-Lymphocytes cytology
B-Lymphocytes immunology
Cell Line
Humans
Immunoglobulin M biosynthesis
Lymphocyte Activation
Plasma Cells cytology
T-Lymphocytes cytology
Tetanus Toxoid pharmacology
Epitopes
T-Lymphocytes immunology
T-Lymphocytes, Helper-Inducer metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2980
- Volume :
- 12
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 6180903
- Full Text :
- https://doi.org/10.1002/eji.1830120604