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Histidine dissimilation in Streptomyces coelicolor.
- Source :
-
Journal of general microbiology [J Gen Microbiol] 1982 Sep; Vol. 128 (9), pp. 2029-40. - Publication Year :
- 1982
-
Abstract
- A growth technique that allows strains of Streptomyces coelicolor to grow dispersed in defined liquid medium has been devised and used to determine the pathway of histidine degradation by S. coelicolor. Enzymic, chromatographic and stoichiometric analyses indicated that histidine is dissimilated via N-formyl-L-glutamic acid. The enzymes for histidine utilization (hut) are induced when histidine or urocanate is included in the culture medium. Biochemical evidence suggested that urocanate, or a further metabolite, is the physiological inducer. Three hut mutants were isolated and characterized. Two of the mutants exhibit an uninducible phenotype, whereas the third mutant appears to be defective in the structural gene for formiminoglutamate iminohydrolase. Haploid recombinant analysis was employed to locate all three mutations in the left empty region of the chromosomal map.
- Subjects :
- Amidohydrolases biosynthesis
Chromosome Mapping
Crosses, Genetic
Culture Media
Enzyme Induction
Formiminoglutamic Acid metabolism
Histidine Ammonia-Lyase biosynthesis
Hydrolases biosynthesis
Hydrolases metabolism
Mutation
Streptomyces genetics
Streptomyces growth & development
Urocanate Hydratase biosynthesis
Urocanic Acid metabolism
Bacterial Proteins
Histidine metabolism
Streptomyces metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1287
- Volume :
- 128
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of general microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 6129283
- Full Text :
- https://doi.org/10.1099/00221287-128-9-2029