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Isoenzyme characterization of chloroquine-resistant isolates of Plasmodium falciparum from Burma.
- Source :
-
Transactions of the Royal Society of Tropical Medicine and Hygiene [Trans R Soc Trop Med Hyg] 1984; Vol. 78 (4), pp. 471-3. - Publication Year :
- 1984
-
Abstract
- Sixty isolates of Plasmodium falciparum collected from hospital in-patients in Rangoon and out-patients from villages near Rangoon were tested in vitro for chloroquine sensitivity and then cultured to carry out starch gel electrophoresis of the following parasite enzyme: glucose phosphate isomerase (GPI) (EC.5.3.1.9.), NADP-dependent glutamate dehydrogenase (GDH) EC.1.4.1.4), lactate dehydrogenase (LDH) (EC.1.1.1.27) and 6-phosphogluconate dehydrogenase (6PGD) (E.C.1.1.1.44). Variations were observed in three (GPI, GDH and LDH) of the four enzymes examined. Three forms of parasite GPI, two forms of parasite GDH, two forms of parasite LDH and only one form of parasite 6PGD were identified. Enzyme polymorphism was more common in isolates with higher resistance to chloroquine. One isolate with parasite GDH-2 and six isolates with parasite LDH-2 were recorded from chloroquine-resistant cases.
- Subjects :
- Animals
Drug Resistance, Microbial
Electrophoresis, Starch Gel
Glucose-6-Phosphate Isomerase analysis
Glutamate Dehydrogenase analysis
Glutamate Dehydrogenase (NADP+)
L-Lactate Dehydrogenase analysis
Myanmar
Phosphogluconate Dehydrogenase analysis
Plasmodium falciparum drug effects
Chloroquine pharmacology
Isoenzymes analysis
Plasmodium falciparum enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0035-9203
- Volume :
- 78
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Transactions of the Royal Society of Tropical Medicine and Hygiene
- Publication Type :
- Academic Journal
- Accession number :
- 6091301
- Full Text :
- https://doi.org/10.1016/0035-9203(84)90064-6