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Properties of hydroxysteroid oxidoreductase isolated from yeast.
- Source :
-
Acta microbiologica Academiae Scientiarum Hungaricae [Acta Microbiol Acad Sci Hung] 1975; Vol. 22 (4), pp. 463-71. - Publication Year :
- 1975
-
Abstract
- Yeasts can advantageously be utilized for the production of the 17beta-hydroxy-derivative, from 3-methoxy-8,14-seco-1,3,5(10),9(11)-estratetraene-14,17-dione (14,17-dione) while 14alpha-hydroxy and 14alpha,17beta-dihydroxy-derivatives are also formed. The biochemical properties of yeasts' enzymes responsible for the formation of the two monohydroxy-derivatives have been studied in detail. In the cell-free extract of Saccharomyces the presence of two hydroxysteroid oxidoreductases could be detected. The first enzyme forms 3beta,17beta-dihydroxy-derivative from 5alpha-androstane-317-dione. This enzyme is responsible for the formation of 17beta-hydroxy-derivative from 14,17-dione. The second enzyme forms 3alpha-hydroxy-derivative from 5beta-androstanedione as well as 14alpha-hydroxy-derivative from its 14,17-dione. The cofactor of both enzymes is pyridine nucleotide. The two enzymes possessing different properties can selectively be inhibited.
Details
- Language :
- English
- ISSN :
- 0001-6187
- Volume :
- 22
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Acta microbiologica Academiae Scientiarum Hungaricae
- Publication Type :
- Academic Journal
- Accession number :
- 5855