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The evaluation of a radioimmunoassay for phenothiazines and thioxanthenes using an iodinated tracer.
- Source :
-
Journal of immunological methods [J Immunol Methods] 1979; Vol. 31 (1-2), pp. 159-66. - Publication Year :
- 1979
-
Abstract
- Antisera have been raised in rabbits to the immogen 2-trifluoromethylphenothiazine-10-beta-propionate bovine serum albumin. An [125I]-labelled tyrosine methyl ester derivative of the immunogen precursor has been synthesised and used with the antisera to develop a simple, precise and sensitive radioimmunoassay for phenothiazines and thioxanthenes bearing 2-trifluoromethyl substituents. The assay can detect 0.4 ng/ml of fluphenazine, trifluorperazine or (Z)-flupenthixol in 100 microliter of human serum without interference from their sulphoxide or 7-hydroxylated metabolites. An acceptable correlation between this assay and an established fluphenazine radioimmunoassay using commercial [3H]fluphenazine has been obtained for plasma samples.
Details
- Language :
- English
- ISSN :
- 0022-1759
- Volume :
- 31
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 512372
- Full Text :
- https://doi.org/10.1016/0022-1759(79)90296-5