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Antigenic heterogeneity among pyocins of Pseudomonas aeruginosa.
- Source :
-
Infection and immunity [Infect Immun] 1975 Aug; Vol. 12 (2), pp. 318-23. - Publication Year :
- 1975
-
Abstract
- We investigated the effect of FeSO4 on phagocytosis-associated, increased oxidative metabolism via the hexose monophosphate shunt, with special attention to its effect on H2O2 levels. The availability of glutathione peroxidase and glutathione reductase for H2O2 disposal and hexose monophosphate shunt stimulation also are evaluated. The results show an impairment of phagocytosis-associated hexose monophosphate shunt activity together with an increase both of resting and phagocytosing formate oxidation. These apparently paradoxical findings are resolved by demonstrating a direct enhancement of formate oxidation by FeSO4 in a cell-free system. In addition, measurement of H2O2 concentrations via scopoletin fluorescence shows reduction of H2O2 by FeSO4. There is no effect on either glutathione peroxidase or glutathione reductase activities. These data suggest that one mechanism of FeSO4 impairment of microbicidal activity is by its removal of H2O2.
- Subjects :
- Absorption
Animals
Antigens, Bacterial
Female
Immune Sera
Male
Pseudomonas Infections immunology
Pseudomonas aeruginosa growth & development
Pseudomonas aeruginosa metabolism
Pyocins metabolism
Rabbits immunology
Serotyping
Structure-Activity Relationship
Bacteriocins immunology
Epitopes
Pseudomonas aeruginosa immunology
Pyocins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 12
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 51006
- Full Text :
- https://doi.org/10.1128/iai.12.2.318-323.1975