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Chemical composition of the cell wall of the H37Ra strain of Mycobacterium tuberculosis.
- Source :
-
Journal of bacteriology [J Bacteriol] 1970 Jun; Vol. 102 (3), pp. 733-9. - Publication Year :
- 1970
-
Abstract
- The cell wall of the H37Ra strain of Mycobacterium tuberculosis was isolated and freed of extraneous noncovalently linked material by a series of extraction and enzymatic procedures. Chemical analysis of the cell wall has revealed the following composition: 22.8% amino acids, principally alanine, glutamate, and diaminopimelate in a molar ratio of 1:1.8:0.8; 24.7% reducing sugars, all in the form of arabinose and galactose in a molar ratio of 2.6:1; and 3.95% amino sugars, all in the form of glucosamine, muramic acid, and galactosamine in a molar ratio of 1:6.6:0.8. About 32.1% of the dry weight of the cell wall is lipid, of this about 55% is in the form of two series of mycolic acids. Each series of mycolic acids contains two homologues differing by 28 mass units. One pair of homologues contains in each a carbonyl function and an unsaturated double bond; the other pair contains two cyclopropane groups in each homologue. The remaining lipids are composed principally of normal saturated fatty acids, including tuberculostearic acid.
- Subjects :
- Alanine analysis
Arabinose analysis
Autoanalysis
Chemical Phenomena
Chemistry
Chromatography, Gas
Chromatography, Ion Exchange
Electrophoresis
Fatty Acids analysis
Galactose analysis
Glucosamine analysis
Glutamates analysis
Microscopy, Electron
Microscopy, Electron, Scanning
Paper
Pimelic Acids analysis
Spectrophotometry
Amino Acids analysis
Amino Sugars analysis
Cell Wall analysis
Lipids analysis
Monosaccharides analysis
Mycobacterium tuberculosis analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 102
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 4988039
- Full Text :
- https://doi.org/10.1128/jb.102.3.733-739.1970