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Metabolic consequences of a block in the synthesis of 5-keto-D-fructose in a mutant of Gluconobacter cerinus.
- Source :
-
Journal of bacteriology [J Bacteriol] 1974 Aug; Vol. 119 (2), pp. 363-70. - Publication Year :
- 1974
-
Abstract
- A mutant of Gluconobacter cerinus var. ammoniacus, IFO 3267, has been isolated which is deficient with respect to fructose 5-dehydrogenase, the enzyme catalyzing the oxidation of d-fructose to 5-keto-d-fructose (5 KF). Growth of this mutant on fructose as the sole carbon source was impaired unless the culture medium was supplemented with 5 KF. Significant randomization of the 1 and 6 positions of fructose has been reported previously for the wild-type organism during growth on this ketohexose. The pattern of (3)H incorporation into the C5 position of ribonucleic acid-ribose when the mutant was grown on [1-(3)H]fructose and [6-(3)H]fructose in the presence of 5 KF indicated that such randomization did not occur in this variant. The randomization observed in the wild type is, therefore, a consequence of the partial oxidation of fructose to the symmetrical 5 KF intermediate prior to its conversion to pentose. When the mutant was grown on [1-(3)H]fructose in the presence of unlabeled 5 KF, [5-(3)H]fructose appeared in the culture medium. Thus, 5 KF served as the oxidant for the nicotinamide adenine dinucleotide phosphate, reduced form, generated during growth on fructose.
- Subjects :
- Alcohol Oxidoreductases metabolism
Bacteria enzymology
Carbon Radioisotopes
Cell-Free System
Chromatography, Paper
Fructose metabolism
Glucose-6-Phosphate Isomerase metabolism
Mutagens
Nitrosoguanidines
Ribose biosynthesis
Stereoisomerism
Tritium
Alcohol Oxidoreductases biosynthesis
Bacteria metabolism
Ketoses biosynthesis
Mutation
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 119
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 4853173
- Full Text :
- https://doi.org/10.1128/jb.119.2.363-370.1974