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Isolation of a bisulfite reductase activity from Desulfotomaculum nigrificans and its identification as the carbon monoxide-binding pigment P582.
- Source :
-
Journal of bacteriology [J Bacteriol] 1973 Oct; Vol. 116 (1), pp. 392-6. - Publication Year :
- 1973
-
Abstract
- Crude preparations of Desulfotomaculum nigrificans were found to reduce bisulfite to trithionate, thiosulfate, and sulfide. The bisulfite reductase of this organism was partially purified and observed to reduce bisulfite to trithionate as the major product and with thiosulfate and sulfide as minor products. The enzyme exhibited spectral properties identical to the carbon monoxide-reacting pigment (P582) isolated from this organism. It is concluded that the bisulfite reductase of D. nigrificans is P582 and that this organism utilizes a pathway which involves trithionate during the reduction of bisulfite to sulfide.
- Subjects :
- Ammonium Sulfate
Bacteria metabolism
Carbon Monoxide metabolism
Cell-Free System
Chemical Precipitation
Chromatography, Paper
Colorimetry
Electrophoresis, Polyacrylamide Gel
Oxidation-Reduction
Oxidoreductases analysis
Oxidoreductases isolation & purification
Oxidoreductases metabolism
Pigments, Biological metabolism
Protein Binding
Spectrophotometry
Sulfates biosynthesis
Sulfides biosynthesis
Sulfites metabolism
Thiosulfates biosynthesis
Bacteria enzymology
Pigments, Biological analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 116
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 4745421
- Full Text :
- https://doi.org/10.1128/jb.116.1.392-396.1973