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Cell sorting in the presence of cytochalasin B.
- Source :
-
The Journal of cell biology [J Cell Biol] 1972 Dec; Vol. 55 (3), pp. 542-53. - Publication Year :
- 1972
-
Abstract
- The ability of cytochalasin B to inhibit ruffled membrane activity and cellular locomotion of vertebrate cells in monolayer culture prompted its use to study the necessity for this kind of active cellular locomotion in cell sorting in heterotypic cell aggregates. Cell sorting was inhibited in chick embryo heart-pigmented retina aggregates but a remarkable degree of sorting did occur in neural retina-pigmented retina aggregates. In these experiments, the levels of cytochalasin B employed (5 or 10 microg/ml) are sufficient to inhibit completely locomotion of these cell types in monolayer culture. It is proposed that the degree of cell movement achieved during sorting in neural retina-pigmented retina aggregates in the presence of cytochalasin B is the result of changes in cell contact resulting from adhesive interaction of cells. The effect of cytochalasin B on the initial aggregation of dissociated cells was also tested. With the cell types used in this study (chick embryo neural retina and limb bud), aggregation was not affected for a period of several hours.
- Subjects :
- Animals
Cell Aggregation drug effects
Cell Differentiation
Cells, Cultured
Chick Embryo
Cytochalasin B pharmacology
Extremities drug effects
Extremities embryology
Heart Ventricles embryology
Myocardium cytology
Retina cytology
Retina embryology
Cell Movement drug effects
Epithelial Cells
Epithelium drug effects
Heart Ventricles drug effects
Indoles pharmacology
Mitosporic Fungi
Retina drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 55
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 4676368
- Full Text :
- https://doi.org/10.1083/jcb.55.3.542