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Phospholipid composition and metabolism of Micrococcus denitrificans.
- Source :
-
Journal of bacteriology [J Bacteriol] 1972 Dec; Vol. 112 (3), pp. 1288-94. - Publication Year :
- 1972
-
Abstract
- The phospholipid composition of Micrococcus denitrificans was unusual in that phosphatidyl choline (PC) was a major phospholipid (30.9%). Other phospholipids were phosphatidyl glycerol (PG, 52.4%), phosphatidyl ethanolamine (PE, 5.8%), an unknown phospholipid (5.3%), cardiolipin (CL, 3.2%), phosphatidyl dimethylethanolamine (PDME, 0.9%), phosphatidyl monomethylethanolamine (PMME, 0.6%), phosphatidyl serine (PS, 0.5%), and phosphatidic acid (0.4%). Kinetics of (32)P incorporation suggested that PC was formed by the successive methylations of PE. Pulse-chase experiments with pulses of (32)P or acetate-1-(14)C to exponentially growing cells showed loss of isotopes from PMME, PDME, PS, and CL with biphasic kinetics suggesting the same type of multiple pools of these lipids as proposed in other bacteria. The major phospholipids, PC, PG, and PE, were metabolically stable under these conditions. The fatty acids isolated from the complex lipids were also unusual in being a simple mixture of seven fatty acids with oleic acid representing 86% of the total. Few free fatty acids and no non-extractable fatty acids associated with the cell wall or membrane were found.
- Subjects :
- Autoradiography
Carbon Isotopes
Cardiolipins analysis
Cell Membrane analysis
Chromatography, Gas
Chromatography, Thin Layer
Electron Transport
Fatty Acids analysis
Fatty Acids, Nonesterified analysis
Glycerol analysis
Lipids analysis
Lipids isolation & purification
Paracoccus denitrificans analysis
Paracoccus denitrificans growth & development
Paracoccus denitrificans metabolism
Phosphatidylcholines analysis
Phosphatidylethanolamines analysis
Phospholipids metabolism
Phosphorus Isotopes
Micrococcus analysis
Phospholipids analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 112
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 4640503
- Full Text :
- https://doi.org/10.1128/jb.112.3.1288-1294.1972