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Characteristics of streptolysin O hemolysis: kinetics of hemoglobin and 86rubidium release.
- Source :
-
Infection and immunity [Infect Immun] 1974 Jun; Vol. 9 (6), pp. 1022-7. - Publication Year :
- 1974
-
Abstract
- The characteristics of hemolysis produced by streptolysin O (SLO) were investigated in rabbit, human, and rat erythrocytes. Kinetic studies of hemoglobin (Hb) release showed that rabbit and human erythrocytes exhibited typical "multi-hit" survival curves. Extrapolation of these curves to the ordinate indicated that approximately two molecules of SLO may be sufficient to produce lysis of a single cell. In contrast, exponential ("single-hit") survival curves were observed when rat erythrocytes were treated with SLO. At 0 C, high concentrations of SLO rapidly lysed rabbit erythrocytes; low concentrations had no effect on the cells at this temperature. The release of intracellular (86)rubidium ((86)Rb(+)) and Hb in rabbit erythrocytes exposed to SLO was investigated. In the presence of phosphate-buffered saline, rubidium and Hb were lost at the same rate from toxin-treated cells. The addition of bovine serum albumin to the suspending medium did not retard the escape of Hb, and the efflux of (86)Rb(+) only slightly preceded Hb loss. Addition of sucrose to the cells delayed both Hb and rubidium release. These results are interpreted as indicating that the "colloid-osmotic" lytic process is not involved in the hemolysis of erythrocytes by streptolysin O.
- Subjects :
- Animals
Erythrocytes drug effects
Humans
Rabbits
Radioisotopes
Rats
Rubidium metabolism
Serum Albumin, Bovine pharmacology
Spectrophotometry
Streptococcus pyogenes metabolism
Streptolysins isolation & purification
Sucrose pharmacology
Erythrocytes metabolism
Hemoglobins metabolism
Hemolysis drug effects
Streptolysins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 9
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 4598253
- Full Text :
- https://doi.org/10.1128/iai.9.6.1022-1027.1974