Isolation of covalently closed circular deoxyribonucleic acid from bacteria which produce exocellular nuclease.

Authors :: Timmis K
Winkler U
Source :: Journal of bacteriology [J Bacteriol] 1973 Jan; Vol. 113 (1), pp. 508-9.
Publication Year :: 1973
Abstract: Reproducible yields of covalently closed circular (plasmid) deoxyribonucleic acid were obtained from mutants defective for extracellular nuclease but not from the corresponding wild-type strain of Serratia marcescens

Subjects :: Carbon Isotopes
Cell-Free System
Centrifugation, Density Gradient
DNA Replication
DNA, Bacterial analysis
DNA, Bacterial biosynthesis
DNA, Circular isolation & purification
Endonucleases metabolism
Extrachromosomal Inheritance
Mutation
Serratia marcescens enzymology
Serratia marcescens metabolism
Tritium
DNA, Bacterial isolation & purification
Endonucleases biosynthesis
Serratia marcescens analysis

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