Back to Search
Start Over
Purification, properties, and regulation of glutamic dehydrogenase of Bacillus licheniformis.
- Source :
-
Journal of bacteriology [J Bacteriol] 1971 May; Vol. 106 (2), pp. 375-85. - Publication Year :
- 1971
-
Abstract
- Cell-free extracts of Bacillus licheniformis and B. cereus were found to contain high specific activities of nicotinamide adenine dinucleotide phosphate (NADP)-dependent-l-glutamate dehydrogenase [EC 1.4.1.4; l-glutamate: NADP oxidoreductase (deaminating)]. Maximum specific activities were found in extracts of cells during the late exponential phase of growth when ammonium ion served as the sole source of nitrogen. Extremely low specific activities were detected throughout the growth cycle when l-glutamate or Casamino Acids served as the source of carbon and nitrogen. The enzyme was purified 55-fold from crude extracts of B. licheniformis, and apparent kinetic constants were determined. Sigmoidal saturation kinetics were not observed, and various adenylates had no effect on the enzyme. Repression of enzyme synthesis during growth on l-glutamate or Casamino Acids was partially overcome by additions of glucose or pyruvate, and this apparent derepression was totally abolished by inhibitors of ribonucleic acid and protein synthesis. Similarly, additions of l-glutamate or Casamino Acids to cells growing on glucose-ammonium ion resulted in strong repression of enzyme synthesis. It is suggested that the enzyme serves an anabolic role in metabolism. Nicotinamide adenine dinucleotide-dependent glutamate dehydrogenase activity was not detected in five species of Bacillus, irrespective of nutritional conditions or of the physiological age of cells.
- Subjects :
- Amino Acids metabolism
Ammonium Chloride metabolism
Bacillus growth & development
Bacillus metabolism
Cell-Free System
Chemical Precipitation
Chloramphenicol pharmacology
Chromatography, DEAE-Cellulose
Colorimetry
Culture Media
Enzyme Activation
Enzyme Repression
Glucose metabolism
Glutamates metabolism
Malates metabolism
Mitomycins pharmacology
NADP metabolism
Quaternary Ammonium Compounds
Rifampin pharmacology
Solvents
Species Specificity
Spectrophotometry
Sulfates
Bacillus enzymology
Glutamate Dehydrogenase biosynthesis
Glutamate Dehydrogenase isolation & purification
Glutamate Dehydrogenase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 106
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 4396790
- Full Text :
- https://doi.org/10.1128/jb.106.2.375-385.1971