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Comparative transport activity of intact cells, membrane vesicles, and mesosomes of Bacillus licheniformis.
- Source :
-
Journal of bacteriology [J Bacteriol] 1973 Jan; Vol. 113 (1), pp. 329-40. - Publication Year :
- 1973
-
Abstract
- Sodium ion was shown to stimulate strongly the transport of l-glutamic acid into cells of Bacillus licheniformis 6346 His(-). Lithium ion had a slight capacity to replace Na(+) in this capacity, but K(+) was without effect. Three of five amino acids tested. l-glutamic acid, l-aspartic acid, and l-alanine, were concentrated against a gradient in the cells. Intracellular pools of these amino acids were extractable with 5% trichloroacetic acid. Pools of l-histidine and l-lysine could not be detected. No evidence of active transport of lysine into cells could be detected, and histidine was taken up in the absence of chloramphenicol but not in its presence. The uptake of glutamic acid by membrane vesicle preparations was strongly stimulated by reduced nicotinamide adenine dinucleotide (NADH) and to a lesser extent by succinate. The presence of phenazine methosulfate increased uptake in the presence of succinate. Either l- or d-lactate and adenosine triphosphate were without effect. None of these compounds stimulated the uptake of glutamic acid by mesosomes, although some mesosome preparations contained separable membrane which was very active. NADH strongly stimulated the uptake of aspartic acid and alanine by membrane vesicles but had only a slight effect on the uptake of histidine and lysine. No evidence of active transport of any of the amino acids into mesosomes could be detected either in the presence or absence of NADH. NADH stimulation of the uptake of glutamic acid by membrane vesicles was destroyed by exposure to light of 360 nm; this inactivation was reversible by vitamin K(2(5)) or K(2(10)). Sodium ion stimulated transport of glutamic acid by membrane vesicles.
- Subjects :
- Alanine metabolism
Aspartic Acid metabolism
Bacillus growth & development
Biological Transport, Active drug effects
Carbon Isotopes
Cell Fractionation
Cell Membrane Permeability
Centrifugation, Density Gradient
Chromatography, Paper
Culture Media
Glutamates metabolism
Histidine metabolism
Light
Lysine metabolism
NAD pharmacology
Sodium pharmacology
Stereoisomerism
Succinates pharmacology
Vitamin K pharmacology
Amino Acids metabolism
Bacillus metabolism
Cell Membrane metabolism
Inclusion Bodies metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 113
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 4347247
- Full Text :
- https://doi.org/10.1128/jb.113.1.329-340.1973