Back to Search
Start Over
An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes.
- Source :
-
The Journal of cell biology [J Cell Biol] 1973 Jan; Vol. 56 (1), pp. 191-205. - Publication Year :
- 1973
-
Abstract
- A cell fractionation procedure is described which allows the preparation from rat liver of a rough microsome population containing almost 50% of the membrane-bound ribosomes of the tissue. The fraction is not contaminated with free ribosomes or smooth microsomes, and, by various other criteria, is suitable for studies of ribosome-membrane interaction.
- Subjects :
- Amino Acids metabolism
Animals
Cell Fractionation
Centrifugation, Density Gradient
DNA analysis
Electron Transport Complex IV analysis
Endoplasmic Reticulum analysis
In Vitro Techniques
Liver enzymology
Male
Methods
Microsomes, Liver analysis
Microsomes, Liver metabolism
Phospholipids analysis
Proteins analysis
RNA analysis
Rats
Spectrophotometry, Ultraviolet
Sucrose
Time Factors
Tritium
Cell Membrane
Liver cytology
Ribosomes
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 56
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 4345164
- Full Text :
- https://doi.org/10.1083/jcb.56.1.191