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Mode of action of oxathiin systemic fungicides. V. Effect on electron transport system of Ustilago maydis and Saccharomyces cerevisiae.
- Source :
-
Journal of bacteriology [J Bacteriol] 1972 May; Vol. 110 (2), pp. 628-32. - Publication Year :
- 1972
-
Abstract
- The systemic fungicide carboxin (5,6-dihydro-2-methyl-1,4-oxathiin-3-carboxanilide) at 100 mum inhibited succinate cytochrome c reductase in mitochondria from Ustilago maydis and Saccharomyces cerevisiae. It did not have any effect on reduced nicotinamide adenine dinucleotide (NADH) cytochrome c reductase. Succinate coenzyme Q reductase was also inhibited, but NADH coenzyme Q reductase was not. When dichlorophenolindophenol (DCIP) was used as the terminal acceptor of electrons from the oxidation of succinate, carboxin was very effective in inhibiting succinate-DCIP reductase. Carboxin was inhibitory to succinic dehydrogenase assayed with phenazine methosulfate plus DCIP when intact mitochondria were used as the enzyme source but not when solubilized enzyme was used. The main site of action of carboxin, therefore, appears to lie between succinate and coenzyme Q. The dioxide analogue of carboxin was also effective in inhibiting succinate-cytochrome c reductase, succinate-coenzyme Q reductase, or succinate-DCIP reductase, whereas the monoxide analogue was less effective in inhibiting these enzymes.
- Subjects :
- Anilides pharmacology
Basidiomycota drug effects
Basidiomycota enzymology
Cell Fractionation
Coenzymes antagonists & inhibitors
Indophenol
Mitochondria enzymology
NAD
Oxathiins pharmacology
Oxidoreductases antagonists & inhibitors
Oxidoreductases metabolism
Saccharomyces cerevisiae drug effects
Saccharomyces cerevisiae enzymology
Saccharomyces cerevisiae metabolism
Spectrophotometry
Succinate Dehydrogenase antagonists & inhibitors
Succinate Dehydrogenase metabolism
Succinates
Ubiquinone
Basidiomycota metabolism
Electron Transport drug effects
Fungicides, Industrial pharmacology
Heterocyclic Compounds pharmacology
Saccharomyces metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 110
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 4336692
- Full Text :
- https://doi.org/10.1128/jb.110.2.628-632.1972