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Effect of 4-azaleucine upon leucine metabolism in Salmonella typhimurium.
- Source :
-
Journal of bacteriology [J Bacteriol] 1971 Oct; Vol. 108 (1), pp. 95-104. - Publication Year :
- 1971
-
Abstract
- dl-4-Azaleucine (5 x 3(-3)m) added to exponentially growing cells of Salmonella typhimurium resulted in an abrupt cessation of growth lasting 4 to 8 hr followed by a resumption of division. The transitory nature of inhibition was not due to the instability or modification of the analogue or to a derepression of leucine-forming enzymes. Of many compounds tested, leucine served most efficiently to reverse 4-azaleucine-induced inhibition. Inhibition of growth can be explained by the fact that 4-azaleucine inhibits alpha-isopropylmalate synthase, the first enzyme unique to leucine biosynthesis. The analogue was a poor inhibitor of both the transamination of alpha-ketoisocaproate to leucine and the charging of leucine to transfer ribonucleic acid. With a leucine auxotroph starved for leucine, the analogue was incorporated into protein specifically in place of leucine. Such incorporation was accompanied by the death of almost all of the cells.
- Subjects :
- Amino Acyl-tRNA Synthetases metabolism
Bacterial Proteins biosynthesis
Bacteriological Techniques
Carbon Isotopes
Cell Division
Cell-Free System
Chromatography
Colorimetry
Culture Media
Dicarboxylic Acids
Gels
Leucyl Aminopeptidase metabolism
Lyases antagonists & inhibitors
Lyases metabolism
Lysine metabolism
Methylamines pharmacology
Oxidoreductases metabolism
Propionates pharmacology
Salmonella growth & development
Salmonella typhimurium drug effects
Salmonella typhimurium enzymology
Silicon Dioxide
Amino Acids pharmacology
Leucine metabolism
Salmonella typhimurium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 108
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 4330744
- Full Text :
- https://doi.org/10.1128/jb.108.1.95-104.1971