A quantitative comparison of formation of spontaneous and virus-produced viable hybrids.

Authors :: Coon HG
Weiss MC
Source :: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1969 Mar; Vol. 62 (3), pp. 852-9.
Publication Year :: 1969
Abstract: Ultraviolet-inactivated Sendai virus used in conjunction with a selective system reproducibly gives high yields of viable hybrid cell lines. With two different crosses, the frequency of hybrid colonies obtained after virus treatment was found to be 100 times greater than the frequency of spontaneous hybrids, and this increase was found to show little variation between 33 and 1000 hemagglutinating units of virus. No differences have been found between the properties of hybrids obtained after Sendai treatment and those obtained from untreated mixed cultures of parental cells.

Subjects :: Animals
Cell Line
Clone Cells
Connective Tissue
Culture Media
L Cells
Liver
Mice
Parainfluenza Virus 1, Human radiation effects
Radiation Genetics
Rats
Ultraviolet Rays
Culture Techniques
Hybridization, Genetic

Language :: English
ISSN :: 0027-8424
Volume :: 62
Issue :: 3
Database :: MEDLINE
Journal :: Proceedings of the National Academy of Sciences of the United States of America
Publication Type :: Academic Journal
Accession number :: 4308097
Full Text :: https://doi.org/10.1073/pnas.62.3.852

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