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Isolation and properties of selenomethionine-resistant mutants of Neurospora crassa.
- Source :
-
Genetics [Genetics] 1974 Aug; Vol. 77 (4), pp. 627-38. - Publication Year :
- 1974
-
Abstract
- Mutants resistant to selenomethionine were isolated, and their properties studied. Mapping studies indicate that the mutation sites are located near the eth-1(r) locus in linkage group I, about ten map units away from the mating type locus. The sites of new mutation are either allelic to or very close to eth-1(r). They are resistant not only to selenomethionine but also to ethionine, while the ethionine-resistant mutant, eth-1(r), is sensitive to selenomethionine. The selenomethionine-resistant mutants are also temperature-sensitive mutants. However, they can grow at higher temperatures in medium containing 1 M glycerol.-It is very unlikely that the resistance is due to a change in the permeability of the membrane. Aryl sulfatase of se-met(r) mutants is not repressed by a high concentration of methionine (5 mM), although inorganic sulfate (2 mM) still can cause total repression. The gamma-cystathionase levels of the mutants are normal, but the S-adenosylmethionine synthetase levels are only one-tenth of that observed in the wild-type strain. The heat-stability of this enzyme in the mutant is also different from that of the wild-type enzyme suggesting that the mutation might affect the structural gene of S-adenosylmethionine synthetase.
- Subjects :
- Biological Transport
Cell Membrane Permeability
Chromosome Mapping
Crosses, Genetic
Ethionine pharmacology
Genes
Genetic Linkage
Hot Temperature
Hydro-Lyases metabolism
L-Serine Dehydratase metabolism
Ligases metabolism
Methionine pharmacology
Neurospora crassa enzymology
Neurospora crassa isolation & purification
S-Adenosylmethionine
Drug Resistance, Microbial
Extrachromosomal Inheritance
Methionine analogs & derivatives
Mutation
Neurospora drug effects
Neurospora crassa drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0016-6731
- Volume :
- 77
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 4278470
- Full Text :
- https://doi.org/10.1093/genetics/77.4.627