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Distribution of repetitious sequences in chick nuclear DNA.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 1974 Feb; Vol. 1 (2), pp. 309-22. - Publication Year :
- 1974
-
Abstract
- By an improved method of hydroxylapatite chromatography, the reassociated sequences of chick nuclear DNA were isolated, and their base composition analysed. By increasing the amount of reassociation, the G + C content of the renatured sequences decreased progressively to reach a mean value corresponding to that of the total DNA. In order to study the distribution of the families, or group of families having different amount of reassociation, DNA was fractionated by CsC1 density gradient centrifugation. Fractions having different G + C content were obtained, and their reassociation rates analysed. At high C(o)t value of renaturation (C(o)t=50) the amount of reassociated sequences included in the high or in the low buoyant density DNA fractions was approximately the same, but their G + C content was as expected different. At lower C(o)t values of renaturation (between C(o)t of 0.2 and the C(o)t of 10), the results indicated an heterogeneity of the repeated sequences in the A + T rich DNA fractions, as compared to the G + C rich ones.
- Subjects :
- Animals
Base Sequence
Binding Sites
Centrifugation, Density Gradient
Chickens
Chromatography, Ion Exchange
Deoxyribonucleotides analysis
Edetic Acid
Electrophoresis, Paper
Evaluation Studies as Topic
Fibroblasts analysis
Fibroblasts cytology
Hydrogen-Ion Concentration
Hydroxyapatites
Kinetics
Methods
Nucleic Acid Conformation
Nucleic Acid Denaturation
Nucleic Acid Renaturation
Phosphorus Radioisotopes
Sodium Dodecyl Sulfate
Temperature
Cell Nucleus analysis
DNA analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0305-1048
- Volume :
- 1
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 4213036
- Full Text :
- https://doi.org/10.1093/nar/1.2.309