Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture.

Authors :: Klement V
Rowe WP
Hartley JW
Pugh WE
Source :: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1969 Jul; Vol. 63 (3), pp. 753-8.
Publication Year :: 1969
Abstract: Tissue culture cells of a Rous virus-induced rat tumor undergo syncytium formation when placed in contact with mouse embryo cell cultures infected with murine leukemia viruses. This phenomenon can be used as a cytopathic end point for isolating and titrating these viruses in tissue culture. The principle should be applicable to detection of leukemia viruses of other species.

Subjects :: Animals
Biological Assay
Cell Line
Culture Media
Culture Techniques
Methods
Mice
Rats
Staining and Labeling
Avian Sarcoma Viruses isolation & purification
Cytopathogenic Effect, Viral
Leukemia Virus, Murine isolation & purification
Virus Cultivation

Language :: English
ISSN :: 0027-8424
Volume :: 63
Issue :: 3
Database :: MEDLINE
Journal :: Proceedings of the National Academy of Sciences of the United States of America
Publication Type :: Academic Journal
Accession number :: 4186808
Full Text :: https://doi.org/10.1073/pnas.63.3.753

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