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Induction of type C viruses in cultured guinea pig cells.
- Source :
-
Journal of virology [J Virol] 1973 Jul; Vol. 12 (1), pp. 177-87. - Publication Year :
- 1973
-
Abstract
- Particles morphologically resembling type C viruses were activated by bromodeoxyuridine (BUdR; 10(-4) M) treatment of cultured guinea pig cells. Virus particles were isolated from the cells of normal and leukemic strain 2 and random-bred guinea pigs (adult and embryonic). Immature virus particles with electron-lucent cores were found in the cytoplasmic matrix. The mature particles with electron-dense cores were found outside the cells and some appeared in the process of budding from the plasma membrane. The peak of virus production was observed within 4 days of BUdR treatment. When compared to the amount of virus produced in darkness, visible light enhanced virus production, whereas exposure of BUdR-treated cells to UV light either had no effect or inhibited virus production. Virus particles had a density of 1.16 gm/ml, possessed an oncornavirus-specific reverse transcriptase, and contained a large-molecular-weight RNA (65-70S) which dissociated into 36S subunits after heat denaturation. The BUdR-activated virus particles, therefore, possessed the morphological, biophysical, and biochemical characteristics of type C oncornaviruses.
- Subjects :
- Animals
Bromodeoxyuridine pharmacology
Cell Membrane microbiology
Cells, Cultured
Centrifugation, Density Gradient
Cytoplasm microbiology
Guinea Pigs
Inclusion Bodies, Viral
Kidney
Leukemia, Experimental
Light
Microscopy, Electron
Molecular Weight
Muscles
RNA, Viral analysis
RNA-Directed DNA Polymerase metabolism
Radiation Effects
Retroviridae analysis
Retroviridae enzymology
Retroviridae growth & development
Spleen
Ultraviolet Rays
Virus Replication drug effects
Virus Replication radiation effects
Oncogenic Viruses growth & development
RNA Viruses growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 4125158
- Full Text :
- https://doi.org/10.1128/JVI.12.1.177-187.1973