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Properties of hamster embryo fibroblasts transformed in vitro after exposure to ultraviolet-irradiated herpes simplex virus type 2.
- Source :
-
Journal of virology [J Virol] 1971 Oct; Vol. 8 (4), pp. 469-77. - Publication Year :
- 1971
-
Abstract
- An in vitro method which led to the transformation of hamster embryo fibroblasts after exposure to herpes simplex virus type 2 (HSV-2) inactivated with ultraviolet irradiation is described. The transformed cells (333-8-9) produced tumors when inoculated into newborn Syrian hamsters but not when injected into weanling Syrian hamsters of the same LSH inbred strain. However, after one in vivo passage, the 333-8-9 cells became highly oncogenic in weanling hamsters. No infectious virus was recovered from these cells. Herpes simplex virus antigens were detected in the transformed cells by the indirect immunofluorescence technique. Sera from tumor-bearing hamsters contained antibody with highly specific neutralizing activity against HSV-2. These studies indicate the continued involvement of the HSV-2 genome in an oncogenic cell line.
- Subjects :
- Animals
Antibodies analysis
Antibody Specificity
Antigens, Viral isolation & purification
Cells, Cultured immunology
Cells, Cultured microbiology
Cricetinae
Culture Techniques
Cytopathogenic Effect, Viral
Cytoplasm immunology
Fluorescent Antibody Technique
Genetics, Microbial
Humans
Immune Sera
Inclusion Bodies, Viral
Kidney
Neoplasm Transplantation
Neoplasms, Experimental blood
Neoplasms, Experimental etiology
Neoplasms, Experimental immunology
Neoplasms, Experimental microbiology
Neutralization Tests
Rabbits
Radiation Effects
Simplexvirus immunology
Simplexvirus radiation effects
Staining and Labeling
Trypsin
Cell Transformation, Neoplastic
Fibroblasts
Simplexvirus pathogenicity
Ultraviolet Rays
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 8
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 4108570
- Full Text :
- https://doi.org/10.1128/JVI.8.4.469-477.1971