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Expression of the vasopressin and oxytocin genes in human hypothalami.
- Source :
-
FEBS letters [FEBS Lett] 1985 Nov 25; Vol. 193 (1), pp. 12-6. - Publication Year :
- 1985
-
Abstract
- Poly(A)+ RNA isolated from post-mortem human hypothalami has been used to characterize the poly-protein precursors to vasopressin and oxytocin. Translation in a cell-free system and subsequent immuno-precipitation with antibodies raised against either vasopressin or neurophysin identified a product of Mr 19000 (prepro-vasopressin). A second less intense product of Mr 16500 was tentatively identified as prepro-oxytocin. A cDNA library derived from the human hypothalamic poly(A)+ RNA was screened for vasopressin and oxytocin-encoding cDNA using heterologous probes; clones encoding the two precursors were identified and found to be organized as their rat and bovine counterparts. Northern blot analysis shows that the mRNAs for the two prepro-hormones consist of approximately 840 (AVP) and approximately 700 (OT) nucleotides.
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 193
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 4065330
- Full Text :
- https://doi.org/10.1016/0014-5793(85)80069-7