Back to Search
Start Over
Inhibitory effect of phenothiazine derivatives on bone in vivo and osteoblastic cells in vitro.
- Source :
-
Biochemical pharmacology [Biochem Pharmacol] 1985 Nov 01; Vol. 34 (21), pp. 3885-9. - Publication Year :
- 1985
-
Abstract
- We tested the inhibitory effects of phenothiazine derivatives on bone in vivo and osteoblastic cells in vitro. Chlorpromazine (CPZ) and trifluoperazine (TFPZ) dose-dependently decreased alkaline phosphatase activity in calvariae of rats: half-maximal inhibitory effects of CPZ and TFPZ were at 2.0 and 4.0 mg/kg, respectively. These effects were more specific for calvaria and ileum than for liver and duodenum. CPZ inhibited the proliferation of osteoblastic clone MC3T3-E1 cells to a greater extent than that of liver epithelial clone RLC-18(4) cells in vitro. CPZ, TFPZ and perphenazine (PNZ) also affected rather specifically alkaline phosphatase activity and collagen synthesis and were not cytotoxic. These in vivo and in vitro findings suggest inhibitory effects on osteoblastic cell function(s). However, promethazine (PMZ) had little effect in vivo and in vitro. In addition, increases in serum calcium and phosphate induced by CPZ indicate its possible involvement in bone resorption.
- Subjects :
- Alkaline Phosphatase antagonists & inhibitors
Animals
Bone and Bones metabolism
Calcium blood
Collagen biosynthesis
In Vitro Techniques
Osteoblasts metabolism
Rats
Rats, Inbred Strains
Structure-Activity Relationship
Bone and Bones drug effects
Osteoblasts drug effects
Phenothiazines pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-2952
- Volume :
- 34
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Biochemical pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 4062963
- Full Text :
- https://doi.org/10.1016/0006-2952(85)90439-3