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Wound tumor virus polypeptide synthesis in productive noncytopathic infection of cultured insect vector cells.

Wound tumor virus polypeptide synthesis in productive noncytopathic infection of cultured insect vector cells.

Authors :
Peterson AJ
Nuss DL
Source :
Journal of virology [J Virol] 1985 Nov; Vol. 56 (2), pp. 620-4.
Publication Year :
1985

Abstract

Inoculation of the leafhopper cell line AC-20 with wound tumor virus resulted in a productive noncytopathic infection with no detectable alteration of cellular protein synthesis. Virus-specific polypeptide synthesis, detectable by 8 h postinoculation, increased in a linear fashion, reaching a peak (approximately 10 to 15% of total protein synthesis) by 48 h postinoculation. The rate of viral protein synthesis continued at this level for several days but declined, relative to cellular protein synthesis, as infected cells were passaged. By passage 10, the synthesis of viral polypeptides was reduced to a level approximately 5% of that observed at 48 h postinoculation. Viral protein synthesis was not stimulated by superinfection. Viral antigens and infectious virus persisted in the majority (greater than 90%) of cells in an infected culture even after more than 100 passages. The synthesis of wound tumor virus polypeptides in infected insect vector cells appears to be regulated in a coordinated and selective manner.

Details

Language :
English
ISSN :
0022-538X
Volume :
56
Issue :
2
Database :
MEDLINE
Journal :
Journal of virology
Publication Type :
Academic Journal
Accession number :
4057360
Full Text :
https://doi.org/10.1128/JVI.56.2.620-624.1985