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[Characterization of the "4 S-trypsin" form of the cytosoluble estradiol receptor of calf uterus in a nondenaturing system].
- Source :
-
Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles [C R Acad Hebd Seances Acad Sci D] 1977 Jan 10; Vol. 284 (2), pp. 137-40. - Publication Year :
- 1977
-
Abstract
- The characterisation of the "4 S-trypsin" form of the estradiol-receptor from calf uterus cytosol was carried out by polyacrylamide gel electrophoresis under non-denaturing conditions. In a multiphasic buffer system (upper buffer Tris-glycine pH25degrees C = 8,6, lower buffer Tris HCl pH25degrees C = 7,4), three radioactive estradiol peaks were observed. The first was free estradiol, the second estradiol initially complexed with a macromolecule and dissociated during electrophoresis, and the third the hormone-receptor complex. The sedimentation coefficient (4 S) of this complex was the same before and after electrophoresis. Its mean geometric radius is R = 2.53 + 0.08 nm and its molecular weight was estimated to be 55,000.
Details
- Language :
- French
- Volume :
- 284
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles
- Publication Type :
- Academic Journal
- Accession number :
- 402239