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Product inhibition of aminopyrine N-demethylation in isolated hepatocytes.
- Source :
-
Journal of pharmacobio-dynamics [J Pharmacobiodyn] 1985 Mar; Vol. 8 (3), pp. 193-8. - Publication Year :
- 1985
-
Abstract
- The product inhibition of aminopyrine (AM) N-demethylation by 4-monomethylaminoantipyrine (MAA) was examined in vitro in preparations of isolated rat hepatocytes using gas chromatograph-mass spectrometer (GC-MS). The kinetic study for AM N-demethylation, which was determined by MAA formation, was studied at concentrations from 0.2 to 4.0 mM with or without various concentrations of D3-MAA. D3-MAA inhibited AM N-demethylation non-competitively at concentrations of 0.2 to 1.0 mM of AM (Ki = 0.81 mM), though it did competitively at concentrations of 1.0 to 4.0 mM of AM (Ki = 0.81 mM). From the present observation, it was suggested that the inhibition type of AM N-demethylation by D3-MAA was dependent on the substrate (AM) concentration.
- Subjects :
- Aminopyrine metabolism
Animals
Dealkylation
Dipyrone analogs & derivatives
Dipyrone pharmacology
Gas Chromatography-Mass Spectrometry
In Vitro Techniques
Indicators and Reagents
Kinetics
Male
Rats
Rats, Inbred Strains
Aminopyrine N-Demethylase antagonists & inhibitors
Liver enzymology
Pyrazolones
Subjects
Details
- Language :
- English
- ISSN :
- 0386-846X
- Volume :
- 8
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of pharmacobio-dynamics
- Publication Type :
- Academic Journal
- Accession number :
- 4009411
- Full Text :
- https://doi.org/10.1248/bpb1978.8.193