Preparation, separation and identification of novel hypocholesterolemic peptides from wheat germ: An in vitro and in silico study.

The aim of this study was to prepare, isolate, and identify hypocholesterolemic peptides from wheat germ protein and explore their efficacy. Wheat germ protein was hydrolyzed using four commercial enzymes. Hydrolysate, with the highest in vitro hypocholesterolemic activity was isolated using ultrafiltration and macroporous resin. The fractions with highest binding affinity to sodium taurocholate were evaluated for cholesterol-lowering activity and resistance to digestion using Caco-2 monolayers. Fraction III had the highest cholesterol-lowering activity, reducing the subcutaneous transport and absorption of cholesterol and resisted digestion. Nano-LC-MS/MS and molecular docking were used to identify cholesterol-lowering peptides from Fraction III. Three cholesterol-lowering peptides, FAAGAPP, GAGDIPGGIG, and GPVPDTGIFS, were identified. These peptides exhibited cholesterol micelle solubility, specifically by 76.2 %, 68.3 %, and 64.7 %, respectively. In summary, wheat germ peptides exhibited significant cholesterol-lowering activity in vitro, suggesting their potential for application in functional foods.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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