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Targeted APT8(16-34) obtained by cell-SELEX and its internalization with miR-23-5p into activated hepatic stellate cells.

Authors :
Yang X
Huang L
Yang LQ
Wu SY
Huang L
Wang JJ
Li BT
Wang Y
Wang XL
Ni YR
Zhang RT
Zhang YQ
Zhang HB
Zhang BQ
Ma L
Wu JF
Jiang CL
Source :
Hepatology international [Hepatol Int] 2024 Dec 16. Date of Electronic Publication: 2024 Dec 16.
Publication Year :
2024
Publisher :
Ahead of Print

Abstract

Background: The activation of hepatic stellate cells play a pivotal role in the pathogenesis of hepatic fibrosis. However, the current lack of specifically identified targets on these cells poses a significant challenge in developing targeted delivery tools for effective anti-hepatic fibrosis therapeutics in clinical practice.<br />Methods: Cell-systematic evolution of ligands by exponential enrichment method was conducted on HSC-T6 cell line to screen out activated hepatic stellate cell-specific aptamers. The specificity of the selected aptamers in targeting hepatic stellate cells was confirmed after truncation optimization. Furthermore, the optimal aptamer was conjugated with miR-23b-5p via C6 linkage to evaluate the targeting specificity of this complex and assess its potential in downregulating liver fibrosis-related proteins and slowing down the progression of liver fibrosis.<br />Results: The present study successful identified 11 highly enriched single-stranded DNA sequences (APT1-11) that specifically target activated hepatic stellate cells. Subsequent affinity detection and optimization truncation led to the selection of APT8(16-34), which effectively targeted activated hepatic stellate cells both in vivo and in vitro. Moreover, when conjugated with miR-23b-5p, APT8(16-34) also exhibited internalization ability into activated hepatic stellate cells. The delivered cargo miR-23b-5p by APT8 (16-34) effectively targeted to mRNA, leading to translational inhibition and subsequent downregulation of related proteins.<br />Conclusions: We have identified APT8 (16- 34), which exhibits specific targeting and internalization capabilities into activated hepatic stellate cells. Moreover, when conjugated with miR-23b-5p, APT8 (16-34) also internalizes into activated hepatic stellate cells, enabling miR-23b-5p exert their respective functions.<br />Competing Interests: Declarations. Conflict of interest: The authors declare that there are no conflicts of interests.<br /> (© 2024. Asian Pacific Association for the Study of the Liver.)

Details

Language :
English
ISSN :
1936-0541
Database :
MEDLINE
Journal :
Hepatology international
Publication Type :
Academic Journal
Accession number :
39680325
Full Text :
https://doi.org/10.1007/s12072-024-10760-9