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Simulation of the Chromatography of Oligomers and Polymers with Hypercarb TM Column.

Authors :
Moyses S
Source :
Journal of chromatography. A [J Chromatogr A] 2025 Jan 11; Vol. 1740, pp. 465590. Date of Electronic Publication: 2024 Dec 08.
Publication Year :
2025

Abstract

The retention time of a polymer in liquid chromatography depends on the details of its microstructure and topology. Despite the number of separation modes and methods available for polymers, gaining quantitative information from chromatograms remains a challenge. A model able to predict the LC retention time of a polymer accounting for all possible variations in its microstructure could provide some valuable insight during method development and produce the information necessary to establish unambiguous structure/property relationships. In a previous article, we reported on the separation of end-functionalized oligomers with the Hypercarbâ„¢ column using interaction polymer chromatography. In this article, the chromatograms for the oligomer were simulated using the general model for the partition coefficient of linear polymers in adsorbing pores developed by Gorbunov and Skvortsov [1]. The chromatograms of the oligomer were simulated under a variety of conditions mimicking the experimental ones. The results confirmed the predictive strength of the model. To explain some unexpected results for high molecular weight polymers under size exclusion conditions, hydrodynamic effects were considered as well as a sorbent consisting of two pore networks. This provided new insight into the Hypercarbâ„¢ column properties.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-3778
Volume :
1740
Database :
MEDLINE
Journal :
Journal of chromatography. A
Publication Type :
Academic Journal
Accession number :
39675182
Full Text :
https://doi.org/10.1016/j.chroma.2024.465590