Protocol for feeding strategy and proteomics analysis of zebrafish Danio rerio using S-trap and iTRAQ techniques.

Authors :: Purushothaman K
Kaul D
Rwei Qing SDT
Rocha SDC
Göksu AB
Morales Lange B
Mydland LT
Vij S
Qingsong L
Øverland M
Press CM
Source :: STAR protocols [STAR Protoc] 2024 Dec 10; Vol. 5 (4), pp. 103513. Date of Electronic Publication: 2024 Dec 10.
Publication Year :: 2024
Publisher :: Ahead of Print
Abstract: Yeast serves as a functional alternative and sustainable protein source in aquaculture. This protocol outlines feeding strategy and intestinal proteome analysis of zebrafish (Danio rerio), using S-trap for digestion, iTRAQ, and mass spectrometry for protein quantification. Additionally, it details the analysis of chemical components in feed and functional assessments via Kyoto Encyclopedia of Genes and Genomes (KEGG), Eukaryotic Orthologous Group (KOG), and Gene Ontology (GO). For complete details on the use and execution of this protocol, please refer to Purushothaman et al. <superscript>1</superscript> .<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)

Full Text Access

Tools