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Butea monosperma bark extract: a natural boost for osteogenesis via Wnt/β-catenin pathway activation in adipose-derived mesenchymal stem cells.
Butea monosperma bark extract: a natural boost for osteogenesis via Wnt/β-catenin pathway activation in adipose-derived mesenchymal stem cells.
- Source :
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Biotechnology letters [Biotechnol Lett] 2024 Nov 25; Vol. 47 (1), pp. 3. Date of Electronic Publication: 2024 Nov 25. - Publication Year :
- 2024
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Abstract
- Purpose: To investigate the impact of Butea monosperma (BM) bark extract on the osteogenic differentiation potential of rat adipose-derived mesenchymal stem cells (rADMSCs) and to elucidate the involvement of Wnt/β-catenin pathway in mediating this osseous effect.<br />Methods: Characterizations (antioxidant assays, FTIR and LC/MS analyses) and docking studies (in silico) were performed to evaluate the presence of phytochemicals in the BM extract and their binding capacity to that of the frizzled receptor. rADMSCs were isolated and characterised for its differentiation potential of osteogenesis for stemness. Dose fixation, cytotoxicity, osteogenic differentiation (calcium, mineral deposition, alkaline phosphatase and osteocalcin) and gene expression (osteocalcin, Col1, osteonectin, Bmp2, Runx2, Wnt2, and β-catenin-14 and 28 days) of the extract were also evaluated in vitro.<br />Results: FTIR and LC/MS analyses unveiled the phytochemicals in the extract and with docking studies confirmed their interaction with the frizzled receptor of Wnt/β-catenin pathway. rADMSCs were isolated and differentiated in the presence of the osteogenic induction medium. Dose fixation studies, cytotoxicity and cell viability assessments demonstrated the phytochemicals concentration-dependent cytotoxicity. The presence of specific bone markers highlighted the osteogenic differentiation potential of the phytochemicals. Furthermore, gene expression studies of rADMSCs depicted a heightened bone-forming capacity potentially facilitated by the activation of Wnt/β-catenin pathway.<br />Conclusion:  The phytochemicals of BM promoted the osteogenic differentiation of rADMSCs through the activation of the signalling Wnt/β-Catenin pathway, as evidenced by the significant upregulation of early and late bone markers. The phytochemicals may therefore be positioned as promising therapeutic agents for enhancing bone regeneration, offering new avenues for regenerative medicine.<br />Competing Interests: Declarations. Conflict of interest: Authors have no conflicts to declare. Consent of publication: All authors agree to publish the manuscript in the said journal.<br /> (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
- Subjects :
- Animals
Rats
Molecular Docking Simulation
Adipose Tissue cytology
Adipose Tissue drug effects
Cells, Cultured
Phytochemicals pharmacology
Phytochemicals chemistry
Osteogenesis drug effects
Mesenchymal Stem Cells drug effects
Mesenchymal Stem Cells metabolism
Plant Extracts pharmacology
Plant Extracts chemistry
Plant Bark chemistry
Wnt Signaling Pathway drug effects
Cell Differentiation drug effects
Butea chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6776
- Volume :
- 47
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biotechnology letters
- Publication Type :
- Academic Journal
- Accession number :
- 39585509
- Full Text :
- https://doi.org/10.1007/s10529-024-03545-5