Bacteriological Profile and Antibiogram Pattern of Wound Infection in a Tertiary Care Hospital.

Background and aim Wound infection constitutes a major barrier to healing and can have a harmful effect on the patient's quality of life and also on the healing rate of the wound. The widespread use of antibiotics, along with their extensive history of availability, has resulted in significant issues with resistant organisms contributing to morbidity and mortality. The study was conducted to determine the prevalence of bacterial species obtained from the wound sample and its antibiotic resistance pattern. Materials and methods The cross-sectional observational study was conducted from November 2022 to November 2023 in a tertiary care hospital in Karad. 100 clinical samples of wound swabs and pus were studied. The isolates from these clinical samples were further processed as per the standard prescribed for the identification and antimicrobial susceptibility testing. Results  Out of 100 isolates, 75 were gram-negative and 25 gram-positive. Among the gram-negative isolates, the predominantly isolated organism was  Klebsiella  spp. 20(26.66%), and gram-positive bacteria was mainly Staphylococcus aureus  (23, 92%). Most of the isolates showed sensitivity to vancomycin (20, 86.95%) and resistance to penicillin (18, 78.26%).  Klebsiella  spp. (20, 26.66%) was mostly sensitive to piperacillin/tazobactam (7, 50%), and resistance was seen to ampicillin (12, 85.71%). Conclusion This study showed that Staphylococcus aureus was the most common bacterial isolate causing wound infection, followed by Klebsiella spp. Antimicrobial susceptibility test showed that vancomycin was effective, followed by tetracycline for Staphylococcus aureus and piperacillin/tazobactam, and tigecycline was effective for Klebsiella spp.
Competing Interests: Human subjects: Consent for treatment and open access publication was obtained or waived by all participants in this study. Institutional Ethics Committee Krishna Vishwa Vidyapeeth Deemed to be University, Karad issued approval 066/2021-2022. The Institutional Ethics Committee has hereby given permission to initiate the research project (Protocol Number 066/2021-2022). Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work.
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