Collagen and chitosan-based biogenic sprayable gel of silver nanoparticle for advanced wound care.

Silver nanoparticles have gained significant attention recently due to their unique antibacterial properties, making them promising candidates for wound care applications. This study proposes a novel approach for advanced wound care using a silver nanoparticle-impregnated biogenic spray hydrogel supplemented with collagen and chitosan. Silver nanoparticles were incorporated into the hydrogel (optimized by a QbD approach) to impart antimicrobial activity, crucial for combating wound infections and promoting faster healing. The study assessed the physical and chemical properties of the biogenic hydrogel, including its viscosity, pH, and nanoparticle dispersion characteristics. In vitro, antimicrobial efficacy against common wound pathogens and in vivo studies using chronic wound models in small animals portrayed the immense potential of the developed biogenic hydrogel in effectively reducing the bacterial load of broad-spectrum pathogens. The hydrogel exhibited excellent biocompatibility, supporting cell proliferation and tissue repair without toxic effects. It accelerated wound healing, improved collagen deposition, and enhanced tissue regeneration in the tested animals by reducing proinflammatory cytokines, ROS, and NF-kb levels. Overall, this innovative silver nanoparticle-impregnated biogenic spray hydrogel of collagen and chitosan presents a uniform spray pattern that proved efficient, showing a promising solution for advanced wound care. Its biocompatibility, safety, anti-inflammatory, antimicrobial efficacy, and wound healing properties hold great potential for improving the management of complex wounds, opening new avenues in wound care and regenerative medicine.
Competing Interests: Declarations. Ethical approval: The studies were carried out according to the guidelines of the Council for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Ministry of Social Justice and Empowerment, Government of India. Healthy Wistar rats (170–230 g) of either sex or bred in the animal house of ISF College of Pharmacy-Moga, Punjab, were used in various animal studies. All animal experimentations were carried out as per the approved protocol no. ISFCP/IAEC/CPCSEA/Meeting No. 01/2022/ Protocol No. 01 by the Institutional animal ethical committee (IAEC) formed as per the norms of CPCSEA. Experimental animals were subjected to standard laboratory conditions (i.e., room temperature, 23±2 °C; relative humidity, 55±5%; 12/12 h light/dark cycle) with free access to animal feed and water before experimentation. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)