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Hydrogen-Bond-Induced Melem Assemblies to Resist Aggregation-Caused Quenching for Ultrasensitive ECL Detection of COVID-19 Antigen.

Authors :
Zhu HT
Bao JY
Kang JW
Wang AJ
Yuan PX
Feng JJ
Source :
Analytical chemistry [Anal Chem] 2024 Dec 03; Vol. 96 (48), pp. 19038-19046. Date of Electronic Publication: 2024 Nov 19.
Publication Year :
2024

Abstract

Nowadays, aggregation-caused quenching (ACQ) of organic molecules in aqueous media seriously restricts their analytical and biomedical applications. In this work, hydrogen bond (H-bond) was utilized to resist the ACQ effect of 2,5,8-triamino-1,3,4,6,7,9,9b-heptaazaphenalene (Melem) as an advanced electrochemiluminescence (ECL) luminophore, whose ECL process was carefully studied in an aqueous K <subscript>2</subscript> S <subscript>2</subscript> O <subscript>8</subscript> system coupled with electron paramagnetic resonance (EPR) measurements. Notably, the H-bond-induced Melem assemblies (Melem-H) showed 16.6-fold enhancement in the ECL signals as compared to the Melem aggregates (Melem-A), combined by elaborating the enhanced mechanism. On such basis, the effective ECL signal transduction was in situ achieved through the specific recognition of the double-stranded DNA embedded in Melem-H assemblies (Me-dsDNA) with spike protein (SP) of coronavirus disease 2019 (COVID-19). For that, such an ECL biosensor showed a wider linear range (1.0-125.0 pg mL <superscript>-1</superscript> ) with a lower limit of detection (LOD) down to 0.45 pg mL <superscript>-1</superscript> , which also displayed acceptable results in analysis of human nasal swab samples. Therefore, the work provides a distinctive insight on addressing the ACQ effect and broadening the application scope of the organic emitter and offers a simple platform for biomedical detection.

Details

Language :
English
ISSN :
1520-6882
Volume :
96
Issue :
48
Database :
MEDLINE
Journal :
Analytical chemistry
Publication Type :
Academic Journal
Accession number :
39560124
Full Text :
https://doi.org/10.1021/acs.analchem.4c04016