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In vitro [3H]-erythromycin binding to Staphylococcus aureus.
- Source :
-
Biochemical pharmacology [Biochem Pharmacol] 1986 Mar 15; Vol. 35 (6), pp. 1001-4. - Publication Year :
- 1986
-
Abstract
- Characteristics of erythromycin binding to Staphylococcus aureus were determined by using kinetics and equilibrium binding experiments. Both methods yielded identical values of the dissociation constant, i.e. 0.1 muM. This value was in accord with that found with a bacterial extract of ribosomes which are the organelles where erythromycin exerts its action. This good agreement shows that the dissociation constant of erythromycin determined with intact bacteria is a good reflect of specific bacterial receptors of macrolides, i.e. ribosomes. In addition, mechanism of uptake of the antibiotic by Staphylococcus aureus was investigated. Passive diffusion process was shown to be mainly responsible for this phenomenon.
Details
- Language :
- English
- ISSN :
- 0006-2952
- Volume :
- 35
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biochemical pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 3954790
- Full Text :
- https://doi.org/10.1016/0006-2952(86)90090-0