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A Method for Bioluminescence-Based RNA Monitoring Using Split-Luciferase Reconstitution Techniques.

Authors :
Eguchi M
Yoshimura H
Ozawa T
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2875, pp. 9-20.
Publication Year :
2025

Abstract

A bioluminescence-based RNA monitoring method in living cells was developed using a split NanoLuc (NLuc) reconstitution technique. For specific recognition of a target RNA sequence, a mutant PUM-HD (mPUM) was used. The method was applied to β-actin mRNA in various cells, including primary cultures of rat hippocampal neurons. It allowed for continuous observation of intracellular localization distribution of the target mRNA in living cells, providing insights into various biological phenomena involving intracellular RNA localization and dynamics.<br /> (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Details

Language :
English
ISSN :
1940-6029
Volume :
2875
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
39535636
Full Text :
https://doi.org/10.1007/978-1-0716-4248-1_2