Highly sequence-specific, timing-controllable m 6 A demethylation by modulating RNA-binding affinity of m 6 A erasers.

Recent advancements in tools using programmable RNA binding proteins and m ⁶ A-erasers enable sequence-selective and timing-controllable m ⁶ A demethylation. However, off-target effects are still a concern. This study addresses the problem by reducing the RNA-binding ability of m ⁶ A-erasers. The modulated m ⁶ A-erasers achieved sequence-specific and timing-controllable m ⁶ A demethylation with minimal off-target activity.