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A novel methylation-detection panel for HPV associated high-grade squamous intraepithelial lesion and cervical cancer screening.
- Source :
-
Scientific reports [Sci Rep] 2024 Oct 26; Vol. 14 (1), pp. 25556. Date of Electronic Publication: 2024 Oct 26. - Publication Year :
- 2024
-
Abstract
- Cervical cancer (CC) was considered to be the most common gynaecological cancer, with an estimated 342,000 deaths worldwide each year, as the majority of patients were diagnosed at an advanced stage of the disease. The purpose of this study was to evaluate the predictive value of multi-locus methylation assay for the early detection of CC. The cervical exfoliated cell samples from 492 HPV-positive females with cervical lesions were collected and subjected to methylation detection of gene FAM19A4, EPB41L3 and PAX1 after bisulfite conversion. The levels of gene methylation in patients with different severity of cervical lesions were evaluated and compared. The receiver-operating characteristic (ROC) curve was established and efficacy indexes such as sensitivity, specificity and area under the curve (AUC) were calculated to assess the diagnostic value of DNA methylation detection at multiple gene loci for CC. The methylation levels of FAM19A4, EPB41L3 and PAX1 were significantly increased with the grade of cervical squamous intraepithelial lesions. The sensitivities of FAM19A4, EPB41L3 and PAX1 alone for high-grade squamous intraepithelial lesion (HSIL) and CC diagnosis were 84.6%, 86.3% and 88.0%, respectively; when three markers were combined by a logistic regression model, the sensitivity was 88.0%, with a high specificity of 97.7% and AUC of 0.957 (95% CI 0.937-0.977). Methylation status of FAM19A4, EPB41L3 and PAX1 were highly specific and effective for monitoring the progression of cervical lesions and the tri-gene methylation assay could be used as a triage tool for CC early screening.<br /> (© 2024. The Author(s).)
- Subjects :
- Humans
Female
Middle Aged
Adult
ROC Curve
Biomarkers, Tumor genetics
Squamous Intraepithelial Lesions virology
Squamous Intraepithelial Lesions genetics
Squamous Intraepithelial Lesions diagnosis
Squamous Intraepithelial Lesions pathology
Paired Box Transcription Factors genetics
Squamous Intraepithelial Lesions of the Cervix virology
Squamous Intraepithelial Lesions of the Cervix diagnosis
Squamous Intraepithelial Lesions of the Cervix genetics
Squamous Intraepithelial Lesions of the Cervix pathology
Aged
Papillomaviridae genetics
Papillomaviridae isolation & purification
Sensitivity and Specificity
Uterine Cervical Dysplasia diagnosis
Uterine Cervical Dysplasia virology
Uterine Cervical Dysplasia genetics
Uterine Cervical Dysplasia pathology
Microfilament Proteins
Cytokines
Uterine Cervical Neoplasms diagnosis
Uterine Cervical Neoplasms genetics
Uterine Cervical Neoplasms virology
DNA Methylation
Early Detection of Cancer methods
Papillomavirus Infections diagnosis
Papillomavirus Infections virology
Papillomavirus Infections genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 39462105
- Full Text :
- https://doi.org/10.1038/s41598-024-75047-3