Back to Search
Start Over
The Biological Effect of Enriching the Plasma Content in Platelet-Rich Plasma: An In Vitro Study.
- Source :
-
Biomolecules [Biomolecules] 2024 Oct 18; Vol. 14 (10). Date of Electronic Publication: 2024 Oct 18. - Publication Year :
- 2024
-
Abstract
- Background: Platelet-rich plasma (PRP) formulations have become valuable therapeutic tools in regenerative medicine. In addition, these blood derivates have been successfully included in cell therapy as fetal bovine serum substitutes, due to the real need to avoid the risk of host immunologic reactions and the animal disease transmission associated with reagents from animal origin. However, the protocols for obtaining them should be optimized to improve their biological potential.<br />Methods: PRP-derived preparations with different concentrations of the platelet and plasma components were obtained from the blood of five donors by freeze-drying. Measurements of the pH, protein, and growth factor concentration were performed. Moreover, their biological effects on cell proliferation and migration and their angiogenic potential were assessed.<br />Results: An increased plasma component concentration resulted in an augmented quantity of the total protein content, a significative variation in the hepatocyte growth factor concentration, and an experimental but clinically irrelevant alteration of the pH value. No significant changes were induced in their potential to enhance proliferative and migratory responses in epithelial cells, with the latter being reduced for dermal fibroblasts. The endothelial cell capacity for tube formation was significatively reduced.<br />Conclusions: An increased blood plasma content did not improve the biological potential of the formulations. However, they have emerged as a promising approach for regenerative therapies where neovascularization must be avoided.
- Subjects :
- Humans
Neovascularization, Physiologic
Fibroblasts metabolism
Fibroblasts cytology
Hepatocyte Growth Factor metabolism
Hepatocyte Growth Factor blood
Hydrogen-Ion Concentration
Human Umbilical Vein Endothelial Cells metabolism
Plasma chemistry
Plasma metabolism
Animals
Endothelial Cells metabolism
Endothelial Cells cytology
Platelet-Rich Plasma metabolism
Platelet-Rich Plasma chemistry
Cell Proliferation drug effects
Cell Movement drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2218-273X
- Volume :
- 14
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Biomolecules
- Publication Type :
- Academic Journal
- Accession number :
- 39456261
- Full Text :
- https://doi.org/10.3390/biom14101328