Janibacter alittae sp. nov., an actinobacterium isolated from the gut of marine sandworm ( Alitta virens ).

A novel Gram-stain-positive, facultatively anaerobic, non-motile, catalase-positive, oxidase-negative and ovoid cocci, designated as A1S7 ^T , was isolated from the gut of a marine sandworm ( Alitta virens ). Strain A1S7 ^T exhibited optimal growth at temperatures of 20-30 ℃, pH 6-8 and in the presence of 2-4% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain A1S7 ^T belonged to the genus Janibacter , exhibiting a similarity of 99.0% to Janibacter cremeus KCTC 49873 ^T , followed by Janibacter anophelis KCTC 19282 ^T (98.8%), Janibacter hoylei KCTC 49872 ^T (98.4%), Janibacter limosus KACC 20518 ^T (98.2%) and Janibacter corallicola KACC 21120 ^T (97.2%). The complete genome sequence of strain A1S7 ^T revealed a genome size of 3360920 bp with a genomic G+C content of 70.1 mol%. The orthologous average nucleotide identity and the digital DNA-DNA hybridization values between strain A1S7 ^T and Janibacter cremeus KCTC 49873 ^T were determined to be 89.5 and 37.2%, respectively. The major respiratory quinone was MK-8(H ₄ ). The predominant fatty acids (>10%) included iso -C _16:0 , C _17:1  ω 8 c , C _18:1  ω 9 c and C _17:0 . Polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phosphoglycolipid and three unknown polar lipids. The cell-wall peptidoglycan type was A1 γ . The major whole-cell sugars were ribose, mannose and glucose. Based on phenotypic, phylogenetic, genotypic and chemotaxonomic properties, strain A1S7 ^T represents a novel species in the genus Janibacter , for which the name Janibacter alittae sp. nov. is proposed. The type strain is A1S7 ^T (=KCTC 49714 ^T = JCM 36706 ^T ).