Surgical Technique for Oral Mucosa Harvesting in Autologous Cultivated Oral Mucosal Epithelial Cell Transplantation for Ocular Surface Disorders.

Ex vivo cultivated oral mucosal epithelial cell transplantation (COMET) was first introduced in Japan in June 2021. This technique is used to treat limbal stem cell deficiency (LSCD). This article provides a detailed description of one of the most critical steps in COMET, which is the harvesting of oral mucosa, along with accompanying videos. The samples harvested using this method were successfully cultured into cell sheets, which were then used in surgical procedures without complications.
Competing Interests: Human subjects: Consent was obtained or waived by all participants in this study. The Ethics Committee of Juntendo University Shizuoka Hospital issued approval 886. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work.
(Copyright © 2024, Toshida et al.)