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Nedl1 knockout impaired the learning and memory of mice.
- Source :
-
Physiology & behavior [Physiol Behav] 2024 Oct 16, pp. 114716. Date of Electronic Publication: 2024 Oct 16. - Publication Year :
- 2024
- Publisher :
- Ahead of Print
-
Abstract
- Background: Protein ubiquitination is a common post-translational modification involved in protein degradation and various life processes in cells. NEDL1 is a ubiquitin ligase that is highly expressed primarily in the brain. However, the functions of NEDL1 in social approach/novelty preference, anxiety, learning and memory remain poorly understood.<br />Methods: Nedl1 knockout mice (Nedl1 <superscript>-/-</superscript> ) and wild-type mice (Nedl1 <superscript>+/+</superscript> ) were tested using three-chamber test, elevated plus maze, and Barnes maze. Then, brain tissue was stained, and blood was collected for metabolic analysis.<br />Results: Compared with Nedl1 <superscript>+/+</superscript> mice, Nedl1 <superscript>-/-</superscript> mice showed no differences in social approach/novelty preference and anxiety behavior. Nedl1 <superscript>-/-</superscript> mice displayed impaired learning and memory. Nedl1 knockout did not affect the number of neurons and oligodendrocytes in the hippocampus. Astrocytes proliferated in the hippocampus of Nedl1 <superscript>-/-</superscript> mice. The amino acid metabolism of Nedl1 <superscript>+/+</superscript> and Nedl1 <superscript>-/-</superscript> mice is different, especially the increase in proline and tryptophan.<br />Conclusion: This study showed that Nedl1 knockout impaired learning and memory, which may be related to astrocyte proliferation and amino acid metabolism change. Nedl1 knockout did not affect social style/novelty preference and anxiety behavior in mice. The preliminary study of NEDL1 in neurobehavioral function could help understand the role of NEDL1 in the nervous system.<br />Competing Interests: Declaration of competing interests The authors have no competing interests.<br /> (Copyright © 2024. Published by Elsevier Inc.)
Details
- Language :
- English
- ISSN :
- 1873-507X
- Database :
- MEDLINE
- Journal :
- Physiology & behavior
- Publication Type :
- Academic Journal
- Accession number :
- 39424023
- Full Text :
- https://doi.org/10.1016/j.physbeh.2024.114716