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Physiological, biochemical, and transcriptomic alterations in Castor (Ricinus communis L.) under polyethylene glycol-induced oxidative stress.
- Source :
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BMC plant biology [BMC Plant Biol] 2024 Oct 17; Vol. 24 (1), pp. 973. Date of Electronic Publication: 2024 Oct 17. - Publication Year :
- 2024
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Abstract
- Background: Castor is an important industrial raw material. Drought-induced oxidative stress leads to slow growth and decreased yields in castor. However, the mechanisms of drought-induced oxidative stress in castor remain unclear. Therefore, in this study, physiological, biochemical, and RNA-seq analyses were conducted on the roots of castor plants under PEG-6000 stress for 3 d and 7 d followed by 4 d of hydration.<br />Results: The photosynthetic rate of castor leaves was inhibited under PEG-6000 stress for 3 and 7 d. Biochemical analysis of castor roots stressed for 3 d and 7 d, and rehydrated for 4 d revealed that the activities of APX and CAT were highest after only 3 d of stress, whereas the activities of POD, GR, and SOD peaked after 7 d of stress. RNA-seq analysis revealed 2926, 1507, and 111 differentially expressed genes (DEGs) in the roots of castor plants under PEG-6000 stress for 3 d and 7 d and after 4 d of rehydration, respectively. GO analysis of the DEGs indicated significant enrichment in antioxidant activity. Furthermore, KEGG enrichment analysis of the DEGs revealed significantly enriched metabolic pathways, including glutathione metabolism, fatty acid metabolism, and plant hormone signal transduction. WGCNA identified the core genes PP2C39 and GA2ox4 in the navajowhite1 module, which was upregulated under PEG-6000 stress. On the basis of these results, we propose a model for the response to drought-induced oxidative stress in castor.<br />Conclusions: This study provides valuable antioxidant gene resources, deepening our understanding of antioxidant regulation and paving the way for further molecular breeding of castor plants.<br /> (© 2024. The Author(s).)
- Subjects :
- Plant Roots genetics
Plant Roots metabolism
Plant Roots drug effects
Ricinus genetics
Ricinus physiology
Ricinus metabolism
Gene Expression Regulation, Plant drug effects
Droughts
Ricinus communis genetics
Ricinus communis metabolism
Antioxidants metabolism
Photosynthesis drug effects
Plant Leaves genetics
Plant Leaves metabolism
Plant Leaves drug effects
Gene Expression Profiling
Oxidative Stress
Polyethylene Glycols pharmacology
Transcriptome
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2229
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC plant biology
- Publication Type :
- Academic Journal
- Accession number :
- 39415088
- Full Text :
- https://doi.org/10.1186/s12870-024-05691-4