The TolC and Lipopolysaccharide-Specific Escherichia coli Bacteriophage TLS-the Tlsvirus Archetype Virus.

Authors :: German GJ
DeGiulio JV
Ramsey J
Kropinski AM
Misra R
Source :: PHAGE (New Rochelle, N.Y.) [Phage (New Rochelle)] 2024 Sep 16; Vol. 5 (3), pp. 173-183. Date of Electronic Publication: 2024 Sep 16 (Print Publication: 2024).
Publication Year :: 2024
Abstract: Introduction: TLS is a virulent bacteriophage of Escherichia coli that utilizes TolC and lipopolysaccharide as its cell surface receptors.<br />Methods: The genome was reannotated using the latest online resources and compared to other T1-like phages.<br />Results: The TLS genome consists of 49,902 base pairs, encoding 86 coding sequences that display considerable sequence similarity with the T1 phage genome. It also contains 18 intergenic 21-base long repeats, each of them upstream of a predicted start codon and in the direction of transcription. Data revealed that DNA packaging occurs through the pac site-mediated headful mechanism.<br />Conclusions: Based on sequence analysis of its genome, TLS belongs to the Drexlerviridae family and represents the type member of the Tlsvirus genus.<br /> (© The Author(s) 2024. Published by Mary Ann Liebert, Inc.)

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